Alfred Ashford was an unintended product of his father's, Alexander Ashford's experiments. Alfred was artificially created along with his sister Alexia Ashford, after their father combined the DNA of Veronica Ashford with a human intelligence gene and surrogate egg. Alexia was born with a phenomenally high intellect while Alfred's was at an average high. Growing up idolizing his sister Alexia, he soon grew scorning the rest of the world.
Wandering around the Antarctic facility underground base, Alfred came across a secret hall which could only be open with three gems. One was in Alfred's possession, the other with Alexia and the last with his father. Knowing something special was hidden behind the doors, Alfred manages to retrieve his father's gem and with Alexia's help, the two journeys into the room. In the room they find out the truth behind their birth. Driven insane by the news, the two abducts their father and injects him with the T-Veronica Virus. The virus in turn mutated their father into the horrible monster known as Nosferatu. The twins then hides their father in a room located in the facility.
Noticing the flaws in her creation, Alexia figures that the only way she could become a superior being without mutating into a monster, was to go into a cryo-stasis sleep. After injecting herself with the virus, she had Alfred seal her in the desired stasis in the underground section of the facility.
Resident Evil: Code Veronica
Without the guidance of his sister Alfred became deranged and psychotic. He soon developed another personality where he dressed like his sister and spoke like her. The true breakdown occurred in 1998 when Rockfort Island was attacked, shortly after Claire Redfield was transferred. Arming himself with a sniper rifle, Alfred constantly made attempts to kill Claire and her partner Steve Burnside. In his Alexia state, Alfred attempt to kill the duo, but after seeing himself in the mirror with smeared makeup, he realized he was not his sister. Blaming Claire and Steve, he attempts to kill them again but fails. Ending up in the cryostasic chamber, Alexia awakes and Alfred dies in her arms. Alfred's body is later discovered by Chris Redfield.